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Experimetria Experimetria
PERFUSION SYSTEMS FOR
ISOLATED KIDNEY/LIVER/MESENTERIC BED
These systems are designed for the perfusion of isolated kidney, liver and mesenteric bed of small mammals. The artery of the organ is canulated and perfused with a suitable buffer at either constant pressure or constant flow. Constant pressure is maintained by hydrostatic pressure from a buffer column, and changes in flow are measured. Constant flow is maintained by a precision roller pump, and changes in pressure are measured. Effluents can be collected for further biochemical analysis.

The basic apparatus for model IPO-01 P comes with thermostated tissue bath, electronic stimulator, temperature probe, a column for constant pressure up to 120 mm Hg with tap, a roller pump to fill the column, stand, and rack with power supply. Model IPO-01 F-P also has a precision roller pump for constant flow.
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The organ chamber features its own water bath and heater. The carbogen supply creates vapor which rises through the stainless steel organ support and surrounds the organ with warm moisture to prevent drying. The organ is not immersed.

The clear lid, which keeps the moisture from escaping, has holes to admit perfusion tubing and stimulator electrode. The standard chamber is 52 mm x 41 mm x 16 mm.
COMPLETE SETUPS
IPO-01 P Perfusion apparatus for isolated kidney, liver or mesenteric bed for constant pressure operation. Includes one thermostated tissue bath, one temperature controller (TC), one temperature probe (RBH-01), one column for constant pressure up to 120 mm Hg with tap, one roller pump to fill column, one stand, one rack and power supply
IPO-01 F-P Perfusion apparatus for isolated kidney, liver or mesenteric bed for constant flow operation. Includes one thermostated tissue bath, one temperature controller (TC), one temperature probe (RBH-01), one column for constant pressure up to 120 mm Hg with tap, one roller pump to fill column, one precision roller pump, one stand, one rack and power supply
COMPONENTS/SPARE PARTS
-- Buffer column
-- Overflow glass tube
-- Teflon tap
-- Organ chamber
-- Roller pump to fill column
-- Tubing set (3-pack)
-- Three-way tap (20-pack)
-- Precision roller pump
-- Oxygen bubbler (5-pack)
OHE-02 Heating element
RBH-01 Bath temperature probe
TC Temperature controller
AM-04 Manipulator mounted on ball bearings for TSZ-XX baths. Includes manipulator and electrodes (bi-polar or uni-polar)
-- Cover lid
OPTIONAL DEVICES
-- Circulating water bath (~16 liters)
-- Transonic flow meter with inline flow probe
HG Bridge amplifier for measurement of column pressure
BPR-02 Pressure transducer
ST-01I Single-channel electronic stimulator for pacing. Includes ST-02 Stimulator and ISO-100 isolator
COMPLETE SYSTEM FOR ISOLATED PERFUSED KIDNEY STUDIES
IPO-01 P Perfusion apparatus for isolated kidney, liver or mesenteric bed for constant pressure operation. Includes one thermostated tissue bath, one temperature controller (TC), one temperature probe (RBH-01), one column for constant pressure up to 120 mm Hg with tap, one roller pump to fill column, one stand, one rack and power supply
HG Pressure bridge amplifier to measure perfusion pressure
BPR-01 Reusable blood pressure transducer with dome
AM-04 Manipulator mounted on ball bearings for TSZ-XX baths. Includes manipulator and electrodes (bi-polar or uni-polar)
HAEMOSYS Software and hardware system for hemodynamic data acquisition and analysis. Includes 8-channel interface card (ISY-100), interface cable, computer interface board (ISY-101), rack and power supply (RPW-04), Pentium computer with monitor and mouse
ST-01I Single-channel electronic stimulator for pacing. Includes ST-02 Stimulator and ISO-100 isolator

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